β actin 13e5 rabbit mab (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc β actin 13e5 rabbit mab

TAPC interacts with VEGFR2 and modulates downstream signaling. (a) Cell viability assay of MC38 cells treated with increasing concentrations of TAPC. (b) Immunoblot analysis of VEGFR2 and key regulators of the PI3K–AKT signaling pathway (PI3K, AKT, and STAT3) in MC38 cells treated with PEG-PO or TAPC (5 and 10 μM). <t>β-Actin</t> was used as a loading control. (c) Pull-down assay of VEGFR2 from MC38 cell lysates using biotinylated TAPC, beads-only sample served as control. (d) Confocal IF imaging of MC38 cells incubated with Cy5.5-labeled TAPC and stained for VEGFR2, nuclei counterstained with DAPI. Scale bars: 20 μm. (e) BLI analysis of TAPC binding to recombinant VEGFR2 using serial concentrations (100, 66.7, 44.4, 29.6, 19.8, 13.2, and 8.8 μM). (f) Molecular dynamics simulations showing predicted protein–ligand complexes (top) and binding pocket visualizations (bottom) of VEGFR2 with TAPC, NDMPFI, MBAMF, and TPFE. (g) Binding free energy calculations of these complexes, including van der Waals, electrostatic, solvation, and total energy components. (h) Extracellular acidification rate (ECAR) of MC38 cells treated with control (0 μM), TAPC (2.5 μM), or TAPC (10 μM), with sequential addition of glucose, oligomycin, and 2-deoxyglucose (2-DG). (i) Quantification of glycolysis and glycolytic capacity in MC38 cells treated with control (0 μM), TAPC (2.5 μM), or TAPC (10 μM) (n = 8). Data are presented as mean ± SEM. Statistical significance was assessed using one-way ANOVA with Tukey's multiple comparisons test; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

β Actin 13e5 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B2+actin/pmc12996994-113-35-39
Average 86 stars, based on 1 article reviews

β actin 13e5 rabbit mab - by Bioz Stars, 2026-09

86/100 stars

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1) Product Images from "Aminated fullerene-based nanoplatform enables synergistic VEGFR2-targeted anti-angiogenesis and tumor immunotherapy"

Article Title: Aminated fullerene-based nanoplatform enables synergistic VEGFR2-targeted anti-angiogenesis and tumor immunotherapy

Journal: Bioactive Materials

doi: 10.1016/j.bioactmat.2026.03.016

Figure Legend Snippet: TAPC interacts with VEGFR2 and modulates downstream signaling. (a) Cell viability assay of MC38 cells treated with increasing concentrations of TAPC. (b) Immunoblot analysis of VEGFR2 and key regulators of the PI3K–AKT signaling pathway (PI3K, AKT, and STAT3) in MC38 cells treated with PEG-PO or TAPC (5 and 10 μM). β-Actin was used as a loading control. (c) Pull-down assay of VEGFR2 from MC38 cell lysates using biotinylated TAPC, beads-only sample served as control. (d) Confocal IF imaging of MC38 cells incubated with Cy5.5-labeled TAPC and stained for VEGFR2, nuclei counterstained with DAPI. Scale bars: 20 μm. (e) BLI analysis of TAPC binding to recombinant VEGFR2 using serial concentrations (100, 66.7, 44.4, 29.6, 19.8, 13.2, and 8.8 μM). (f) Molecular dynamics simulations showing predicted protein–ligand complexes (top) and binding pocket visualizations (bottom) of VEGFR2 with TAPC, NDMPFI, MBAMF, and TPFE. (g) Binding free energy calculations of these complexes, including van der Waals, electrostatic, solvation, and total energy components. (h) Extracellular acidification rate (ECAR) of MC38 cells treated with control (0 μM), TAPC (2.5 μM), or TAPC (10 μM), with sequential addition of glucose, oligomycin, and 2-deoxyglucose (2-DG). (i) Quantification of glycolysis and glycolytic capacity in MC38 cells treated with control (0 μM), TAPC (2.5 μM), or TAPC (10 μM) (n = 8). Data are presented as mean ± SEM. Statistical significance was assessed using one-way ANOVA with Tukey's multiple comparisons test; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Techniques Used: Viability Assay, Western Blot, Control, Pull Down Assay, Imaging, Incubation, Labeling, Staining, Binding Assay, Recombinant

In vivo anti-tumor and anti-angiogenic effects of TAPC@CNPs. (a) Schematic illustration of the therapeutic study in Balb/c mice bearing subcutaneous MC38 tumors (n = 7). (b) Body weights of mice during treatment. (c) Photographs of excised tumors collected at endpoint. (d) Tumor growth curves during treatment. Tumor volume was calculated using the formula (length × width 2 )/2. (e) Tumor weights measured at endpoint. (f) Immunoblot analysis of VEGFR2 expression in tumor lysates from different treatment groups, β-actin was used as a reference protein. (g) IHC staining of CD31 in tumor sections from different treatment groups. Scale bar, 100 μm. (h) H&E staining of major organs (heart, liver, spleen, lung, kidney) and tumor tissues. (i) Serum ALT and AST levels measured at endpoint. Data are presented as mean ± SEM. Statistical analysis was performed by one-way ANOVA with Tukey's multiple comparisons test, ns indicates not significant, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001.

Figure Legend Snippet: In vivo anti-tumor and anti-angiogenic effects of TAPC@CNPs. (a) Schematic illustration of the therapeutic study in Balb/c mice bearing subcutaneous MC38 tumors (n = 7). (b) Body weights of mice during treatment. (c) Photographs of excised tumors collected at endpoint. (d) Tumor growth curves during treatment. Tumor volume was calculated using the formula (length × width 2 )/2. (e) Tumor weights measured at endpoint. (f) Immunoblot analysis of VEGFR2 expression in tumor lysates from different treatment groups, β-actin was used as a reference protein. (g) IHC staining of CD31 in tumor sections from different treatment groups. Scale bar, 100 μm. (h) H&E staining of major organs (heart, liver, spleen, lung, kidney) and tumor tissues. (i) Serum ALT and AST levels measured at endpoint. Data are presented as mean ± SEM. Statistical analysis was performed by one-way ANOVA with Tukey's multiple comparisons test, ns indicates not significant, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001.

Techniques Used: In Vivo, Western Blot, Expressing, Immunohistochemistry, Staining

Incubation:

Article Title: Hematopoietic progenitor kinase 1 inhibitor BGB-15025 induces apoptosis in acute myeloid leukemia cells through the cell cycle pathway and mitogen-activated protein kinase/extracellular signal-regulated kinase pathway signaling axis
Article Snippet: .. Membranes were subsequently incubated overnight at 4 °C with specific primary antibodies: β-actin (#4970; 1 : 1000), HPK1 (#46510; 1 : 1000), cyclin D1 (#55506; 1 : 1000), P21 (#2947; 1 : 1000), ERK (#4696; 1 : 1000), phosphorylated ERK (p-ERK, #4370; 1 : 1000), P38 MAPK (#8690; 1 : 1000), and phosphorylated P38 MAPK (p-P38, #9211; 1 : 1000) (all from Cell Signaling Technology, Danvers, Massachusetts, USA). .. Following three 15-min TBST washes, membranes were incubated for 1 h at room temperature with species-matched horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit HS101-01 and goat anti-mouse HS201-01; TransGen Biotech, Beijing, China).

Article Title: Identification of myocardial contractility-related genes as regenerative targets and diagnostic biomarkers in coronary artery disease
Article Snippet: .. After blocking with 5% skimmed milk for 1 h, the membrane was incubated overnight at 4 °C with primary antibodies at 1:1000 dilution: anti-UQCRQ (Cat# ab224180, Abcam), anti-COX7C (Cat# 11411-2-AP, Proteintech), anti-COX6C (Cat# ab150422, Abcam), anti-SLC8A1 (Cat# A03876, BosterBio), anti-COX7A2 (Cat# ab248815, Abcam), anti-TNNT2 (Cat# MA5-12960, ThermoFisher Scientific), anti-CACNB2 (Cat# ab93606, Abcam), anti-CACNB1 (Cat# ab230019, Abcam), and β-actin (Cat# 4967, CST). .. After rinsing with TBST, membranes were incubated with secondary antibodies at 1:2000 dilution: anti-rabbit (Cat# 7074, CST) or anti-mouse (Cat# 7076, CST).

Article Title: Transcriptomics and 4D-DIA proteomic analysis reveal differences in meat quality between Sichuan white rabbits and New Zealand white rabbits
Article Snippet: .. The separated proteins were transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, CA, USA) at a constant voltage of 120 V for 1 h. Membranes were blocked with 5 % bovine serum albumin (BSA) for 1 h at room temperature and incubated overnight at 4 °C with primary antibodies against ACLY (ABMART, Shanghai, China), APMAP (Proteintech, Wuhan, China), and β-ACTIN (Cell Signaling Technology, Boston, US). .. After washing three times with TBST buffer (10 mM Tris-HCl, 150 mM NaCl, 0.1 % Tween-20, pH 7.4), the membranes were incubated with HRP-conjugated secondary antibodies (IgG (H + L); Beyotime Biotechnology, Shanghai, China) for 1 h at room temperature.

Control:

Article Title: Exploring the immunomodulatory effects of environmental contaminants on autoimmune patients: An in vitro approach
Article Snippet: Following washes, membranes were incubated with HRP-conjugated secondary antibodies, and signals were detected using PierceTM ECL substrate (Thermo Scientific, Waltham, MA, USA) on a ChemiDocTM MP imaging system (Bio-Rad, Hercules, CA, USA). .. Membranes were stripped and re-probed for total AKT, NFκB p65, p38 MAPK, STAT1, STAT3, and β-actin (Cell Signaling Technology, Danvers, MA, USA; Santa Cruz Biotechnology, Dallas, TX, USA) as a loading control. ..

Blocking Assay:

Membrane:

Cell Counting:

Article Title: ATM inhibition restores IFN-γ sensitivity and induces ferroptosis in NSCLC via DNA damage response
Article Snippet: IFN-γ was purchased from Biolegend, and ATM inhibitor KU-55933 was purchased from MedChem Express. .. Ferrostatin-1 and Liproxstatin-1 were purchased from Cayman, and the GSH/GSSG Quantification Kit and Cell Counting WST-8 were purchased from Dojindo. γH2AX antibody and β-Actin were purchased from Cell Signaling Technology. .. The human non-small cell lung cancer cell lines PC-9 (kindly gifted from Dr. Kiura, Okayama University, Japan) and A549 (CCL-185, obtained from American Type Culture Collection) were used.

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